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Monkey cell lines

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categories-Monkey cell lines-cryovial-organism-Rhesus macaque-tissue-Kidney-disease–synonyms-Llc-Mk2, LLC-MK-2, LLC-MK2 Original, LLCMK2, LLcMK2, Lilly Laboratories Culture-Monkey Kidney 2-age-Adult-gender–ethnicity–morphology-Epithelial-cellType–growth Properties-Adherent-LLC-MK2 (CLS catalog number 305149)-biosafety Level-1-cultureMedium-Medium 199-mediumSupplements-1% horse serum-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.-freezeMedium-CM-1 (CLS catalog number 800150)