Description

categories-Mouse cell lines-cryovial-organism-Mouse-tissue-Hematopoietic, hybridoma-disease–synonyms-M138, M 138, M-24 (M138), M-24, L138-age–gender–ethnicity–morphology-Round cells-cellType-Lymphoblast-growth Properties-Suspension-L-138 (CLS catalog number 400384)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine, 1 mM sodium pyruvate-subculturing-Start cultures at 2 x 10^5 cells/ml and maintain between 1 x 10^5 and 1 x 10^6 cells/ml. Sub-culture by removing an appropriate amount of cell suspension and place it into new culture flasks already containing fresh cell culture media.-freezeMedium-CM-1 (CLS catalog number 800150)