Description

categories-Reproductive system cancer cell lines-cryovial-organism-Human-tissue-Endocervix-disease-Adenocarcinoma-synonyms-Strain KB-age-30 years-gender-Female-ethnicity-African American-morphology-Epithelial-like-cellType-Epidermoid-growth Properties-Adherent-KB (CLS catalog number 300446)-biosafety Level-1-cultureMedium-EMEM-mediumSupplements-10% FBS, 2 mM L-glutamine, 1% NEAA, 1 mM sodium pyruvate-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 10-15 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 5 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. -freezeMedium-CM-1 (CLS catalog number 800150)