Description

Organism – Human , tissue – Colon descendens, UICC IIIa , disease – Primary adenocarcinoma, TNM stage T3N1M0R0L1V1, grading G3, Lk(n) + 2, Σ Lk(n) 18 , , , synonyms – , – age – 69 years , Male – , Caucasian , – Epithelial-like , – , , Adherent , 1 , – DMEM:Ham’s F12, w: 3.1 g/L Glucose, w: 1.6 mM L-Glutamine, w: 15 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a), – Supplement the medium with 10% FBS , , After thawing, resuspend the cell pellet carefully. Centrifuge at 300 x g for 3 min and discard the supernatant. Seed into 2x 25cm² cell culture flasks and leave the flasks for 48 hrs in the incubator. Replace the spent medium every 2-3 days, until 80-90% confluency is reached. This will take roughly 10-14 days.   – , Every 3 to 5 days – , CM-1 (Cytion catalog number 800100) – , Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes. – – Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.