Description

categories- Leukemia cell lines-cryovial-organism-Human-tissue-Peripheral blood-disease-T-cell acute lymphoblastic leukemia-synonyms-CCRF-CEM C1, CEM-C1, CEM.C1, CEMC1-age-4 years-gender-Female-ethnicity–morphology-Lymphoblast-cellType–growth Properties-Suspension-CEM/C1 (CLS catalog number 305103)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS-subculturing-Resuspend cell suspension in the flask and take representative aliquote to count the cell number per ml. Dilute cell suspension to 1 x 10^5 cells/ml with fresh medium and transfer into new flasks.-freezeMedium-CM-1 (CLS catalog number 800150)