Description

categories-Mouse cell lines-cryovial-organism-Mouse-tissue-Breast-disease-Adenocarcinoma-synonyms–age-1 year-gender-Female-ethnicity–morphology–cellType–growth Properties-Adherent-CDNR4 (CLS catalog number 400391)-biosafety Level-1-cultureMedium-DMEM-mediumSupplements-10% FBS, 2 mM L-glutamine, 4.5 g/L glucose-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 20-30 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium.-freezeMedium-CM-1 (CLS catalog number 800150)