BT-20

Description

categories-Breast cancer cell lines-cryovial-organism-Human-tissue-Breast, mammary gland-disease-Invasive ductal carcinoma-synonyms-BT 20, BT20-age-74 years-gender-Female-ethnicity-Caucasian-morphology-Epithelial-like-cellType–growth Properties-Monolayer, adherent-BT-20 (CLS catalog number 300130)-biosafety Level-1-cultureMedium-DMEM:Hams F12-mediumSupplements-5% FBS, 2 mM L-glutamine-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.-freezeMedium-CM-ACF (CLS catalog number 800650)