Description

categories-Rat cell lines-cryovial-organism-Rat-tissue-Pancreas tumor, exocrine-disease-Neoplasia-synonyms-AR4-2J, AR-42J-age–gender–ethnicity–morphology-Epithelial-like-cellType–growth Properties-Cells grow slowly, in clusters and appear as hollow spheroid colonies. They can pile up and attach loosely.-AR42J (CLS catalog number 500478)-biosafety Level-1-cultureMedium-DMEM-mediumSupplements-10% FBS, 2 mM L-glutamine, 4.5 g/L glucose-subculturing-It is recommended to cover the tissue culture flasks with gelatine prior to cell cultivation. Gelatine is added to the flask, incubated for 30min at 37 ?C and washed once with PBS. Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.-freezeMedium-CM-1 (CLS catalog number 800150)