Description
categories-Mouse cell lines-cryovial-organism-Mouse-tissue-Embryonic-disease–synonyms-3T3 L1, 3T3L1, 3T3-L1 ad, NIH-3T3-L1, NIH3T3-L1, 3T3-Swiss albino-age-Embryo-gender-Male-ethnicity–morphology-Fibroblast-like-cellType-Fibroblast-growth Properties-Adherent-3T3-L1 (CLS catalog number 400103)-biosafety Level-1-cultureMedium-DMEM-mediumSupplements-10% FBS, 2 mM L-glutamine, 4.5 g/L glucose-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.-freezeMedium-CM-1 (CLS catalog number 800150)
