Description

categories- Brain cancer cell lines-cryovial-organism-Human-tissue-Brain-disease-Meningioma, benign cells-synonyms-HBL 52-age-47 years-gender-Female-ethnicity–morphology-Epithelial-like-cellType–growth Properties-Adherent-HBL-52 (CLS catalog number 300188)-biosafety Level-1-cultureMedium-DMEM:Hams F12-mediumSupplements-2% FBS, 2 mM L-glutamine, 4.5 g/L glucose, 15 mM Hepes, 0.00625 mg/ml insulin, 0.00625 mg/ml transferrin, 6.25 ng/ml selenium, 1.25 mg/ml bovine serum albumin, 0.00535 mg/ml linoleic acid, 1x MEM NEAA, 1x MEM Vitamins -subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. -freezeMedium-CM-1 (CLS catalog number 800150)