Description

categories-Head-Neck cancer cell lines-cryovial-organism-Human-tissue-Retina-disease-Retinoblastoma-synonyms-Y79, GM01232, GM01232E-age-2,5 years-gender-Female-ethnicity-Caucasian-morphology-Multicellular clusters -cellType–growth Properties-Clusters in Suspension-Y-79 (CLS catalog number 300382)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine-subculturing-Resuspend cell suspension in the flask and take representative aliquote to count the cell number per ml. Dilute cell suspension to 1×10^5 cells/ml with fresh medium and transfer into new flasks.-freezeMedium-CM-1 (CLS catalog number 800150)