Description

categories-Myeloma cell lines-cryovial-organism-Human-tissue-Plasma cell-disease-Multiple Myeloma-synonyms-U266B1, U266-B1, U266 B1, U-266, U 266, U266S, U266BL, U266-age-53 years-gender-Male-ethnicity–morphology–cellType–growth Properties–U266 (CLS catalog number 300259)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine-subculturing-Start new cultures at 5 x 10^5 viable cells/ml and subculture at 1-2 x 10^6 cells/ml. Prepare dilutions by transferring the appropriate amount of cell suspension into new flasks with fresh medium. Maximum cell density is at 1-2 x 10^6 cell/ml.-freezeMedium-CM-1 (CLS catalog number 800150)