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Leukemia cell lines

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categories- Leukemia cell lines-cryovial-organism-Human-tissue-Spleen-disease–synonyms-TK-6, H2BT-age-5 years-gender-Male-ethnicity–morphology–cellType-Lymphoblast-growth Properties-Suspension-TK6 (CLS catalog number 300357)-biosafety Level-2-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine, 4.5 g/L glucose-subculturing-Start cultures at 2 x 10^5 cells/ml and maintain between 1 x 10^5 and 1 x 10^6 cells/ml. Subculture by transferring an aliquot of the cell suspension into a new cell culture flask already containing an appropriate amount of fresh cell culture medium.-freezeMedium-CM-ACF (CLS catalog number 800650)