Description
categories-Leukemia cell lines-cryovial-organism-Human-tissue-Peripheral blood-disease-Leukemia-synonyms-THP1, THP 1, THPI, O-THP-1, Tohoku Hospital Pediatrics-1-age-1 year-gender-Male-ethnicity–morphology-Round cells-cellType-Monocyte-growth Properties-Suspension-THP-1 (CLS catalog number 300356)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine, 1 mM sodium pyruvate-subculturing-Resuspend the cell suspension in the flask and withdraw a representative aliquot to count the cell number and cell viability. Dilute the cell suspension to 1 x 10^5 viable cells/ml using fresh medium and transfer into new flasks. Keep the flasks in a horizontal position.-freezeMedium-CM-1 (CLS catalog number 800150)
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