Sp2/0-Ag14

Description

categories-Mouse cell lines-cryovial-organism-Mouse-tissue-Blood-disease-B cell hybridoma-synonyms-SP2/0-Ag14, SP2/0-AG14, SP2/0-ag14, Sp2/O-Ag14, SP2/O-Ag14, Sp2/0-Ag-14, SP2-0-Ag14, SP2/0 Ag-14, SP-2/0-AG14, Sp 2/0-Ag 14, Sp2/0, SP2/0, Sp2/O, SP2/O, SP-2, SP2, GM03569, GM3569, GM03569B, GM3569B, GM03569D-age–gender–ethnicity–morphology-Round cells-cellType–growth Properties-Suspension-Sp2/0-Ag14 (CLS catalog number 400481)-biosafety Level-1-cultureMedium-DMEM-mediumSupplements-10% FBS, 2 mM L-glutamine, 4.5 g/L glucose-subculturing-Collect medium with floating cells in a microcentrifuge tube. Rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37 ?C for 10 minutes. Combine the floating cells and the detached cells in one tube, centrifuge at 300xg for 3min. Carefully resuspend the cells in fresh medium and dispense into new flasks which contain fresh medium.-freezeMedium-CM-1 (CLS catalog number 800150)