SH-SY5Y

Description

categories-Neuroblastoma cell lines-cryovial-organism-Human-tissue-Bone Marrow-disease-Neuroblastoma-synonyms-SH-Sy5y, SHSY5Y, SHSY-5Y, SK-SH-SY5Y, SY5Y, SH-SY5Y Parental-age-4 years-gender-Female-ethnicity–morphology-The cells grow as clusters of neuroblastic cells with multiple, short, fine cell processes (neurites). Cells will aggregate, form clumps and float. A confluent monolayer is not formed.-cellType-Neuroblast-growth Properties-Loosely adherent and form clumps at high cell density-SH-SY5Y (CLS catalog number 300154)-biosafety Level-1-cultureMedium-DMEM-mediumSupplements-10% FBS, 2 mM L-glutamine, 4.5 g/L glucose-subculturing-These cells grow as a mixture of floating and adherent cells. Remove the medium with the floating cells, and recover the cells by centrifugation. Rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37 ?C for 10 minutes. Combine with the floating cells recovered above. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium.-freezeMedium-CM-ACF (CLS catalog number 800650)