Description

categories-Reproductive system cancer cell lines-cryovial-organism-Human-tissue-Ovary-disease-Ovarian mixed germ cell tumor-synonyms-PA1, PA I, PAI-age-12 years-gender-Female-ethnicity-Caucasian-morphology-Epithelial-like-cellType–growth Properties-Adherent-PA-1 (CLS catalog number 300402)-biosafety Level-1-cultureMedium-EMEM-mediumSupplements-10% FBS, 2 mM L-glutamine, 1% NEAA, 1 mM sodium pyruvate-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add TrypleExpress (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.-freezeMedium-CM-1 (CLS catalog number 800150)