Description

categories-Mouse cell lines-cryovial-organism-Mouse-tissue-Hematopoietic-disease-Myeloma-synonyms-P3-X63-Ag8.653, P3-X63-Ag8-653, P3-x63-Ag8 653, P3-X63-Ag 8.653, P3-X63Ag8.653, P3-X63.Ag8.653, P3/X63/Ag8.653, P3X63 Ag8.653, P3X63 AG8-653, P3X63-Ag8.653, P3x63-Ag8.653, P3X63 AG 8.653, P3X63Ag8653, P3-X63-Ag8-6-5-3, P3X63Ag8-6-5-3, P3.times.63 Ag8.653, P3.653, X63-Ag 8.6.5.3, X63-AG 8.653, X63-Ag8-653, X63-Ag8.653, X63.Ag8.653, X63Ag8-653, X63Ag8.653, X63AG8.653, P3-653, GM03570, GM3570, GM03570E, NS653-age–gender-Female-ethnicity–morphology-Round cells-cellType–growth Properties-Adherent/suspension-P3X63Ag8.653 (CLS catalog number 400118)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine-subculturing-Subculture by collecting any floating cells in a centrifuge tube. Rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add TrypleExpress (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37 ?C for 10 minutes. Combine all cells, and start new cultures at 4 x 10^5 cells/ml. The cell density should not exceed 2 x 10^6 cells/ml.-freezeMedium-CM-1 (CLS catalog number 800150)