Description

categories-Mouse cell lines-cryovial-organism-Mouse-tissue-Hematopoietic-disease-Mastocytoma-synonyms-P815, P 815-age–gender-Male-ethnicity–morphology-Round cells-cellType-Mast cell-growth Properties-Suspension, some adherent cells-P-815 (CLS catalog number 400242)-biosafety Level-1-cultureMedium-DMEM-mediumSupplements-10% FBS, 2 mM L-glutamine, 4.5 g/L glucose-subculturing-Start cultures at 2 x 10^5 cells/ml and maintain between 1×10^5 and 1×10^6 cells/ml. Subculture by transferring an appropriate aliquot of the suspension into new flasks filled with fresh cell culture medium. Detach adherent cells by washing with PBS first and incubation with Accutase at ambient temeratur 8-10 minutes. Combine with the nonadherent cells and distribute into new cell culture flasks.-freezeMedium-CM-ACF (CLS catalog number 800650)