Description

categories-Mouse cell lines-cryovial-organism-Mouse-tissue-Embryonic-disease–synonyms-NIH3T3-ras, A51, EJ-NIH/3T3, EJ-NIH-3T3-age-Embryo-gender-Male-ethnicity–morphology–cellType-Fibroblast-growth Properties–NIH-3T3-ras (CLS catalog number 400100)-biosafety Level-1-cultureMedium-DMEM-mediumSupplements-10% FBS, 2 mM L-glutamine, 4.5 g/L glucose-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add TrypleExpress (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37 ?C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. Do not allow the cells to become confluent, subculture once per week.-freezeMedium-CM-1 (CLS catalog number 800150)