Description

categories-Lung cancer cell lines-cryovial-organism-Human-tissue-Lung-disease-Lung small cell carcinoma-synonyms-NCI-H-82, H82, H-82, NCI H82, NCIH82, H82sclc-age-41 years-gender-Male-ethnicity-Caucasian-morphology-Epithelial-like-cellType–growth Properties-Aggregates in suspension. The cells grow in very Large aggregates, and the aggregates are the only viable cell population-NCI-H82 (CLS catalog number 300442)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine, 4.5 g/L glucose-subculturing-This line grows as aggregates of cells in suspension. Sub-culture by transferring the cell suspension into new cell culture flasks already filled with the appropriate volume of fresh cell culture medium. Alternatively, the cells may be collected by centrifugation and dispersed into fresh medium.-freezeMedium-CM-1 (CLS catalog number 800150)