Description

categories-Lung cancer cell lines-cryovial-organism-Human-tissue-Lung-disease-Lung small cell carcinoma-synonyms-NCI-H-69, NCI H69, H69, H-69, NCIH69, NCI-HUT-69, H69/P, NCI-H69C, H69C, H69c-age-55 years-gender-Male-ethnicity-Caucasian-morphology–cellType–growth Properties-Floating aggregates-NCI-H69 (H69) (CLS catalog number 300185)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine, 4.5 g/L glucose-subculturing-Allow aggregates to settle to the bottom of the flask, remove and discard the supernatant medium. Add fresh medium, disperse cells by gentle pipetting and dispense into new flasks. Subculture every 6 to 8 days.-freezeMedium-CM-1 (CLS catalog number 800150)