Description

categories-Lung cancer cell lines-cryovial-organism-Human-tissue-Lung-disease-Small cell carcinoma-synonyms-H146, H-146, NCIH146-age-59 years-gender-Male-ethnicity-Caucasian-morphology-Epithelial-like-cellType–growth Properties-Aggregates in suspension-NCI-H146 (CLS catalog number 300182)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine-subculturing-The cells should be subcultured by transferring part of the suspension into fresh new cell culture flasks prefilled with fresh medium. Alternatively, the clusters may be collected by centrifugation and resuspended in fresh medium.-freezeMedium-CM-1 (CLS catalog number 800150)