Description

categories-Lung cancer cell lines-cryovial-organism-Human-tissue-Lung-disease-Pleural mesothelioma-synonyms-MSTO-211 H, MSTO211H, MSTO-211, 211H, MeSoTheliOma-211H-age-62 years-gender-Male-ethnicity-Caucasian-morphology–cellType–growth Properties-Adherent-MSTO-211H (CLS catalog number 300450)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine-subculturing-The cells can reach a saturation density of 400.000 cells per cm2, but will slough off the surface as they attain this density. Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 5 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. -freezeMedium-CM-1 (CLS catalog number 800150)