Description

categories- Leukemia cell lines-cryovial-organism-Human-tissue–disease-Acute lymphoblastic leukemia (ALL)-synonyms-Molt-3, MOLT 3, Molt 3, MOLT3, Molt3-age-19 years-gender-Male-ethnicity-Caucasian-morphology-Round cells-cellType-T lymphocyte-growth Properties-Suspension-MOLT-3 (CLS catalog number 300116)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine, 1% NEAA, 1 mM sodium pyruvate-subculturing-Maintain cultures between 5 x 10^5 to 6.0 x 10^5 cells/ml. Incubate at 5% CO2, 37 ?C until a maximum cell density of 1 – 2 x 10^6 cells/ml is achieved.-freezeMedium-CM-1 (CLS catalog number 800150)