Description

categories- Bone cancer cell lines-cryovial-organism-Human-tissue-Bone-disease-Osteosarcoma-synonyms-MNNG/HOS, MNNG-HOS, HOS-MNNG, HOS/MNNG, MNNGHOS, MNNG/HOS (Cl#5), MNNG/HOS Clone F-5, MNNG, R-1059-D, TE85, Te85, TE-85, HOS-TE85, Hos TE-85, HOS TE 85, HOS TE85, HOS (TE85), HOS(TE85), HOS (TE85, Clone F5), MNNG-HOS (TE 85, clone F-5), TE-85 clone F-5, HOS-Te85, TE 85.T, TE 85 ClF-5, TE-85 clone 5-age-13 years-gender-Female-ethnicity-Caucasian-morphology-Fibroblast-like-cellType–growth Properties-Monolayer, adherent-MNNG-HOS (CL #5) (CLS catalog number 300289)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine, 4.5 g/L glucose-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.-freezeMedium-CM-1 (CLS catalog number 800150)