Description

categories-Bone cancer cell lines-cryovial-organism-Human-tissue-Bone-disease-Osteosarcoma-synonyms-M-G63, MG63-age-14 years-gender-Male-ethnicity-Caucasian-morphology-Fibroblast-like-cellType–growth Properties-Adherent-MG-63 (CLS catalog number 300441)-biosafety Level-1-cultureMedium-DMEM:Hams F12-mediumSupplements-5% FBS, 2 mM L-glutamine-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 5 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. -freezeMedium-CM-ACF (CLS catalog number 800650)