Description
categories-Mouse cell lines-cryovial-organism-Mouse-tissue-Skin-disease-Fibrosarcoma-synonyms-Meth A, Meth-A, Meth-A-sarkom-age-Adult-gender-Female-ethnicity–morphology-Round cells-cellType–growth Properties-Suspension-Meth A sarcoma (CLS catalog number 400284)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine-subculturing-Allow cell aggregates to settle to the bottom of the flask, discard the supernatant medium, disperse the cells with gentle pipetting and dispense into new flasks. Resuspend cell suspension in the flask and take representative aliquot to count the cell number per ml. Dilute cell suspension to 1×10^5 cells/ml with fresh medium and transfer into new flasks.-freezeMedium-CM-1 (CLS catalog number 800150)
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