Description

categories- Avian cell lines-cryovial-organism-Chicken-tissue–disease-Marek disease-synonyms-MDCC MSB1, MDCC-MSB-1, MSB-1, MSB1-age–gender–ethnicity–morphology-Round cells-cellType-Lymphoblast-growth Properties-Suspension-MDCC-MSB1 (CLS catalog number 601413)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine-subculturing-Establish new cultures at 3 x 10^5 viable cells/ml. Maintain the cell density between 1 x 10^5 and 1 x 10^6 cells/ml by transferring an appropriate amount of cell suspension into a new cell culture flask refilled with fresh cell culture medium.-freezeMedium-CM-1 (CLS catalog number 800150)