Description

categories-Mouse cell lines-cryovial-organism-Mouse-tissue-Bone, calvaria-disease–synonyms-MC3T3-E1 SUBCLONE 14-age-Newborn-gender–ethnicity–morphology-Fibroblast-cellType–growth Properties-Adherent-MC3T3-E1 Subclone 14 (CLS catalog number 305185)-biosafety Level-1-cultureMedium-AMEM-mediumSupplements-with ribonucleosides, deoxyribonucleosides, 2 mM L-glutamine, 1 mM sodium pyruvate, without ascorbic acid, 10% FBS-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.-freezeMedium-CM-1 (CLS catalog number 800150)