Description

categories-Intestine cancer cell lines-cryovial-organism-Human-tissue-Colon-disease-Adenocarcinoma-synonyms-Ls174T, LS174t, Ls-174-T, LS-174-T, LS 174 T, LS174T, Ls-174T, LS 174T, LS-174, LS174-age-58 years-gender-Female-ethnicity-Caucasian-morphology-Epithelial-like-cellType–growth Properties-Adherent-LS-174T (CLS catalog number 300392)-biosafety Level-1-cultureMedium-EMEM-mediumSupplements-10% FBS, 2 mM L-glutamin, 1% NEAA-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambiente temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 5 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. -freezeMedium-CM-1 (CLS catalog number 800150)