Description

categories-Rat cell lines-cryovial-organism-Rat-tissue-Testis-disease-Adenoma-synonyms-LC540, LC 540-age-Adult-gender-Male-ethnicity–morphology–cellType-Leydig-growth Properties-Adherent-LC-540 (CLS catalog number 500262)-biosafety Level-1-cultureMedium-EMEM-mediumSupplements-10% FBS, 2 mM L-glutamine, 1% NEAA, 1 mM sodium pyruvate-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37 ?C for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 5 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. -freezeMedium-CM-1 (CLS catalog number 800150)