Description

categories-Mouse cell lines-cryovial-organism-Mouse-tissue-Peripheral blood-disease–synonyms–age–gender–ethnicity–morphology-Lymphoblast-cellType–growth Properties-Suspension-L6565 (CLS catalog number 305189)-biosafety Level-1-cultureMedium-DMEM:Hams F12-mediumSupplements- 2.5 mM L-glutamine adjusted to contain 1.2 g/L sodium bicarbonate, 0.005 mg/ml insulin, 0.01 mg/ml human transferrin, 0.1 mM ethanolamine, 0.1 mM phosphoethanolamine, 25 nM selenium, 500 nM hydrocortisone, 0.005 mM forskolin, bovine pituitary extract (0.15 mg protein per ml)-subculturing-Resuspend cell suspension in the flask and take representative aliquote to count the cell number per ml. Dilute cell suspension to 1 x 10^5 cells/ml with fresh medium and transfer into new flasks.-freezeMedium-CM-1 (CLS catalog number 800150)