Description

categories-Mouse cell lines-cryovial-organism-Mouse-tissue-Connective tissue, normal, subcutaneous, areolar and adipose-disease–synonyms-NCTC clone 929, NCTC 929, NCTC-929, NCTC929, L cell, L cells, L-cell, L-cells, L cell line, L, Strain L-929, L 929, L929, L929(NCTC), Clone 929, Earless cells, Earles L cells-age-100 days-gender-Male-ethnicity–morphology-Fibroblast-like-cellType-Fibroblast-growth Properties-Adherent-L-929 (CLS catalog number 400260)-biosafety Level-1-cultureMedium-DMEM:Hams F12-mediumSupplements-5% FBS, 2 mM L-glutamine, 1 mM sodium pyruvate-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37 ?C for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 5 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. -freezeMedium-CM-1 (CLS catalog number 800150)