Description

categories- Leukemia cell lines-cryovial-organism-Human-tissue-Bone marrow-disease-Acute myelogenous leukemia-synonyms-KG1-age-59 years-gender-Male-ethnicity-Caucasian-morphology–cellType-Myeloblast-growth Properties-Suspension-KG-1 (CLS catalog number 300208)-biosafety Level-1-cultureMedium-IMDM-mediumSupplements-10% FBS, 4 mM L-glutamine-subculturing-Transfer the cell suspension into sterile centrifuge tubes. Collect the cells by spinning down at 300xg for 3 minutes. Discard the supernatant and resuspend the pelleted cells in fresh cell culture medium. Adjust to an optimal cell density between 1 – 3 x 10^5 cells/ml. Split the cells when a maximum cell density of 1 – 2 x 10^6 cells/ml is reached.-freezeMedium-CM-1 (CLS catalog number 800150)