Description

categories- Leukemia cell lines-cryovial-organism-Human-tissue-Blood-disease-Acute myeloblastic leukemia-synonyms-KASUMI-1, Kasumi 1, KASUMI1, Kasumi1-age-7 years-gender-Male-ethnicity-Japanese-morphology-Round cells showing marked variations in both size and nuclear cytoplasmic ratio.-cellType-Myeloblast (AML-acute myeloid leukemia)-growth Properties-Suspension-Kasumi-1 (CLS catalog number 300226)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine-subculturing-Start cultures at 3 x 10^5 cells/ml and split 24 hours later. Subculture the cells in transferring one part of cell suspension into new cell culture flasks already containing an appropriate volume of fresh cell culture medium. Maintain at a cell density between 3 x 10^5 and 6 x 10^5 cells/ml. Viability may drop when the cell density exceeds 1-2 x 10^6 cells/ml.-freezeMedium-CM-ACF (CLS catalog number 800650)