Description
categories-Mouse cell lines-cryovial-organism-Mouse-tissue-Reticulum-disease-Sarcoma-synonyms-J-774A.1, J774A1, J774 A1, J774A.1, J 774A.1, J774 A.1-age-Adult-gender-Female-ethnicity–morphology–cellType-Monocyte-growth Properties-Adherent/suspension-J774A.1 (CLS catalog number 400220)-biosafety Level-1-cultureMedium-DMEM-mediumSupplements-10% FBS, 2 mM L-glutamine, 4.5 g/L glucose-subculturing-Subcultures are to be performed before confluence is reached. otherwise, the cells will round up and detach. Cells can be either detached by scraping or via Accutase. Detachment is facilitated when the monolayer is washed once with PBS and incubated with Accutase for 10 to 15min at ambient temperature. As cells grow semi-adherent, collecting cells from old medium and PBS via centrifugation is recommended. Centrifuge the cell suspension, aspirate the old medium, resuspend the cells in fresh cell culture medium and dispense into new flasks. Using trypsin for detachment is not recommended.-freezeMedium-CM-1 (CLS catalog number 800150)
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