Description

categories-HUVEC-cells-organism-Human-tissue-Umbilical vein-disease–synonyms-HUVEC-p-age–gender–ethnicity-Caucasian-morphology-Endothelial-cellType-Primary cells-growth Properties-Monolayer, adherent-HUVEC, pooled (CLS catalog number 300605)-biosafety Level-Although these HUVEC-p have been tested for the absence of contagious agents, we recommend to strictly follow the appropriate safety precautions. -cultureMedium-ECGM-mediumSupplements–subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. -freezeMedium-CM-1 (CLS catalog number 800150)