Description

categories-Intestine cancer cell lines-cryovial-organism-Human-tissue-Colon recto-sigmoid, UICC IV, Established from a patient-derived xenograft of primary CRC tissue (Colon sigmoid, TNM stage T3N2M1R0L1V1, grade G3, Lk(n) +4, ? Lk(n) 32).-disease-Adenocarcinoma-synonyms-HROC147-age-54 years-gender-Male-ethnicity-Caucasian-morphology-Epithelial-like-cellType–growth Properties-Adherent with special surface, spheroids-HROC147 T0 M1 (CLS catalog number 300856)-biosafety Level-1-cultureMedium-DMEM:Hams F12-mediumSupplements-10% FBS, 2 mM L-glutamine-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add TrypLE Express (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37 ?C for 10 to 15 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. This cell line will result in single cell suspension.-freezeMedium-CM-ACF (CLS catalog number 800650)