Description

categories-Intestine cancer cell lines-cryovial-organism-Human-tissue-Colorectal, Established from a PDX (patient-derived xenograft) of primary CRC tissue (Colon ascendens, TNM stage T2N1M0R0L0V0, grade G2, Lk(n) + 2, ? Lk(n) 23). -disease-Adenocarcinoma-synonyms-HROC103-age-44 years-gender-Male-ethnicity-Caucasian-morphology-Small cells in colonies-cellType-Epithelial -growth Properties-Adherent -HROC103 T0 M1 (CLS catalog number 300802)-biosafety Level-1-cultureMedium-DMEM:Hams F12-mediumSupplements-10% FBS, 2 mM L-glutamine-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add TrypLE Express (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37 ?C for 10 to 15 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. -freezeMedium-CM-ACF (CLS catalog number 800650)