Description

categories-Pancreas cancer cell lines-cryovial-organism-Human-tissue-Pancreas-disease-Pancreatic ductal adenocarcinoma-synonyms-HPAF II, HPAFII, HPAF-2, HPAF2, HPAF/CD18, CD18/HPAF, HPAF-II/CD18, CD-18, CD18, CD 18-age-44 years-gender-Male-ethnicity-European-morphology-Epithelial-cellType–growth Properties-Adherent-HPAF-II (CLS catalog number 305088)-biosafety Level-1-cultureMedium-EMEM-mediumSupplements-10% FBS-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.-freezeMedium-CM-1 (CLS catalog number 800150)