Description
categories-Mouse cell lines-cryovial-organism-Mouse-tissue-Liver-disease-Hepatocellular carcinoma-synonyms-Hepatoma-22, Hepatoma 22-age–gender–ethnicity–morphology-Lymphoblast-cellType–growth Properties-Suspension-H22 (CLS catalog number 305163)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS-subculturing-Resuspend cell suspension in the flask and take representative aliquote to count the cell number per ml. Dilute cell suspension to 1 x 10^5 cells/ml with fresh medium and transfer into new flasks.-freezeMedium-CM-1 (CLS catalog number 800150)
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