Polyclonal antisera from immunized hosts are lipid extracted to improve clarity, salt fractionated, dialyzed against phosphate buffered saline containing sodium azide and freeze-dried. Antisera against whole serums are obtained by immunizing host animals with whole serum. Antisera against whole IgG molecules [i.e. Anti-IgG (H+L)] are recommended for bridging PAP to primary antibodies.-Based on immunoelectrophoresis, the antibody reacts with the F(ab’)2/Fab portion of rabbit IgG. It also reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against the Fc portion of rabbit IgG or against non-immunoglobulin serum proteins. The antibody may cross-react with immunogloublins from other species.