Description

For quantitative determination of glucose oxidase activity and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Use as little as 20 µL samples. Linear detection range in 96-well plate for 20 minute incubation at 25°C: 0.02 to 10 U/L glucose oxidase for colorimetric assays and 0.002 to 1.5 U/L for fluorimetric assays. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD570nm, FL530/585nm. Samples: Cell/tissue lysate, cell culture media etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 0.02 (OD), 0.002U/L (FL).