Description

For quantitative determination of creatinine and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Linear detection range: 4.8 to 500 µM or 0.054-5.7 mg/dL (colorimetric assay) and 0.25 to 100 µM or 0.0028-1.14 mg/dL (fluorimetric assay) for a 60 min reaction. It is 3- and 53-fold more sensitive than the traditional Jaffe method (e.g. DICT-500), especially useful for small samples or where high sensitivity is required. Convenient. The procedure involves adding a single working reagent and reading after 60 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated to process thousands of samples per day. Method: OD570nm; FL530/585nm. Samples: Urine, serum, plasma, and other biological preparations. Species: All. Procedure: 60 min. Size: 100 tests. Detection Limit: Colorimetric assay: 4.8 µM or 0.054 mg/dL; Fluorimetric assay: 0.25 µM or 0.0028 mg/dL.