Description

For sensitive quantitative determination of adipogenesis and high-throughput screening of adipogenesis modulators. Key Features: Sensitive and accurate. Use as little as 40 µL samples. Linear detection range in 96-well plate: 0.16 to 5 nmoles for colorimetric assays and 0.075 to 0.5 nmoles for fluorimetric assays. Fast and convenient. The addition of a single working reagent and 30-min incubation procedure combines sample extraction, hydrolysis and color reaction. The whole procedure is performed at room temperature with NO incubator or heating needed. Robust and amenable to HTS. Homogeneous “mix-incubate-measure” type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD570nm or FL530/585nm. Samples: biological samples (cells, tissues, etc). Species: All. Procedure: 30min. Size: 200 tests.