Description
categories-Lung cancer cell lines-cryovial-organism-Human-tissue-Lung-disease-Carcinoma, azaserine induced-synonyms-DMS 79, DMS79-age-65 years-gender-Male-ethnicity-Caucasian-morphology–cellType–growth Properties-Aggregates in suspension-DMS-79 (CLS catalog number 300164)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine, 4.5 g/L glucose-subculturing-Once or twice a week add 5 ml of fresh cell culture medium, as soon as the culture medium gets acidic. Subculture as soon as many very large clusters are observed. Dissociate the clusters by collecting the cells, rinsing once using PBS without calcium/magnesium and adding 3-5 ml Accutase. Incubate at 37 ?C for 10minutes. Collect the cells following centrifugation, resuspend in fresh cell culture medium and count. Start cultures at 2-4 x 10^4 cells/ml.-freezeMedium-CM-1 (CLS catalog number 800150)
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