Description
categories-Mouse cell lines-cryovial-organism-Mouse-tissue-Blood-disease–synonyms-CTLL 2, CTLL2, CTLL(2)-age–gender–ethnicity–morphology-Single cell suspension, round, shining cells-cellType-Lymphoblast-growth Properties-Suspension-CTLL-2 (CLS catalog number 400482)-biosafety Level-1-cultureMedium-i2Cult-mediumSupplements–subculturing-Immediately after thawing, about 50% viable cells were measured using Trypan Blue dye exclusion. The viability of the cells eventually will drop to even lower values. The cell viability should however increase to |BiggerAs| 80% within 48 hours, at a cell concentration of about 1 million cells/ml. Subculture the cells at an inoculation density of 40000 cells/ml. Control the cell viability every day. Keep the cells at 37 ?C and 5% CO2.-freezeMedium-CM-1 (CLS catalog number 800150)
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