Description

categories- Breast cancer cell lines-cryovial-organism-Human-tissue-Breast, mammary gland-disease-Squamous cell carcinoma-synonyms-COLO-824, COLO 824, Colo 824, COLO #824, COLO824, Colo824, Colorado 824-age-52 years-gender-Female-ethnicity-Caucasian-morphology-Epithelial-like-cellType–growth Properties-Adherent/suspension-Colo-824 (CLS catalog number 300463)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine-subculturing-Collect the non-adherent cells and combine with the slightly adherent cells being knocked off of the bottom of the cell culture vessel.-freezeMedium-CM-1 (CLS catalog number 800150)