Description

categories-Reproductive system cancer cell lines-cryovial-organism-Human-tissue-Cervix-disease-Squamous cell carcinoma-synonyms-Cerv-186, MRI-H-186, MRI-H186-age-42 years-gender-Female-ethnicity-African-morphology-Epithelial-like-cellType–growth Properties-Adherent-CERV-186 (CLS catalog number 300290)-biosafety Level-2-cultureMedium-DMEM:Hams F12-mediumSupplements-5% FBS, 2 mM L-glutamine-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.-freezeMedium-CM-ACF (CLS catalog number 800650)